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ngm agar plates  (Thermo Fisher)


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    Structured Review

    Thermo Fisher ngm agar plates
    Ngm Agar Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ngm+agar+plates/AGAR/pmc12212086-251-20-42
    Average 99 stars, based on 1 article reviews
    ngm agar plates - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: Mitochondrial translocation of TFEB regulates complex I and inflammation.
    Article Snippet: .. Synchronized eggs were incubated on NGM agar plates with E. coli strain OP50 containing 500 nM MitoTracker Deep Red FM (Invitrogen M22426) until day 1 adults. ..

    Article Title: Identification of pararosaniline as a modifier of RNA splicing in Caenorhabditis elegans
    Article Snippet: .. Briefly, age synchronized 1 day of worms were incubated on NGM agar plates with 1 mM of pararosaniline or 0.4% DMSO only control for 24 h. RNA was extracted from n = 4 biological replicates each containing ∼300–500 worms per condition using the Purelink RNA mini kit (12183020; ThermoFisher). .. RNA was then treated with DNaseI (EN0521; ThermoFisher) followed by cDNA synthesis using the Invitrogen Multiscribe reverse transcriptase (4311235; ThermoFisher) with the Applied Biosystems ProFlex Thermocycler.

    Article Title: Identification of pararosaniline as a modifier of RNA splicing in Caenorhabditis elegans.
    Article Snippet: .. Briefly, age synchronized 1 day of worms were incubated on NGM agar plates with 1 mM of pararosaniline or 0.4% DMSO only control for 24 h. RNA was extracted from n = 4 biological replicates each containing ∼300–500 worms per condition using the Purelink RNA mini kit (12183020; ThermoFisher). .. RNA was then treated with DNaseI (EN0521; ThermoFisher) followed by cDNA synthesis using the Invitrogen Multiscribe reverse transcriptase (4311235; ThermoFisher) with the Applied Biosystems ProFlex Thermocycler.

    Article Title: LGG-1/GABARAP lipidation is not required for autophagy and development in Caenorhabditis elegans
    Article Snippet: .. For MitoTracker staining, adult worms were transferred to NGM agar plates containing 3.7 μM of Red CMXRos (Molecular Probes, Invitrogen) and incubated for overnight in the dark. .. One-day adults were transferred to M9 20% BSA (Sigma‐Aldrich, A7030) on 1% phosphatidylcholine (Sigma-Aldrich) pre-coated 200 μm deep flat carriers (Leica Biosystems), followed by cryo‐immobilization in the EMPACT‐2 HPF apparatus (Leica Microsystems; Vienna Austria) as described ( ).

    Article Title: Mitochondrial translocation of TFEB regulates complex I and inflammation
    Article Snippet: .. Synchronized eggs were incubated on NGM agar plates with E. coli strain OP50 containing 500 nM MitoTracker Deep Red FM (Invitrogen M22426 ) until day 1 adults. ..

    Disruption:

    Article Title: Developmental transcriptomics in Pristionchus reveals the environmental responsiveness of a plasticity gene-regulatory network.
    Article Snippet: .. To synchronize worms, we performed cuticular disruption of gravid adults with bleach/NaOH (Stiernagle 2006) and aliquoted eggs to J1s to NGM-agar plates or S-medium liquid culture OP50 E. coli (Werner et al. 2017) or, for t′ =0, directly resuspended in 500 μL TRIzol (Ambion 15596026). ..

    Article Title: Developmental transcriptomics in Pristionchus reveals the environmental responsiveness of a plasticity gene-regulatory network
    Article Snippet: .. To synchronize worms, we performed cuticular disruption of gravid adults with bleach/NaOH ( ) and aliquoted eggs to J1s to NGM-agar plates or S-medium liquid culture OP50 E. coli ( ) or, for t′ = 0, directly resuspended in 500 μL TRIzol (Ambion 15596026). ..

    Article Title: Developmental transcriptomics in Pristionchus reveals the logic of a plasticity gene regulatory network
    Article Snippet: .. To synchronize worms we performed cuticular disruption of gravid adults with bleach/NaOH ( ) and aliquoted eggs-J1s to NGM-agar plates or S-medium liquid culture OP50 E. coli , or for t’=0, directly resuspended in 500 μl Trizole (Ambion cat. #15596026). ..

    Control:

    Article Title: Identification of pararosaniline as a modifier of RNA splicing in Caenorhabditis elegans
    Article Snippet: .. Briefly, age synchronized 1 day of worms were incubated on NGM agar plates with 1 mM of pararosaniline or 0.4% DMSO only control for 24 h. RNA was extracted from n = 4 biological replicates each containing ∼300–500 worms per condition using the Purelink RNA mini kit (12183020; ThermoFisher). .. RNA was then treated with DNaseI (EN0521; ThermoFisher) followed by cDNA synthesis using the Invitrogen Multiscribe reverse transcriptase (4311235; ThermoFisher) with the Applied Biosystems ProFlex Thermocycler.

    Article Title: Identification of pararosaniline as a modifier of RNA splicing in Caenorhabditis elegans.
    Article Snippet: .. Briefly, age synchronized 1 day of worms were incubated on NGM agar plates with 1 mM of pararosaniline or 0.4% DMSO only control for 24 h. RNA was extracted from n = 4 biological replicates each containing ∼300–500 worms per condition using the Purelink RNA mini kit (12183020; ThermoFisher). .. RNA was then treated with DNaseI (EN0521; ThermoFisher) followed by cDNA synthesis using the Invitrogen Multiscribe reverse transcriptase (4311235; ThermoFisher) with the Applied Biosystems ProFlex Thermocycler.

    Staining:

    Article Title: LGG-1/GABARAP lipidation is not required for autophagy and development in Caenorhabditis elegans
    Article Snippet: .. For MitoTracker staining, adult worms were transferred to NGM agar plates containing 3.7 μM of Red CMXRos (Molecular Probes, Invitrogen) and incubated for overnight in the dark. .. One-day adults were transferred to M9 20% BSA (Sigma‐Aldrich, A7030) on 1% phosphatidylcholine (Sigma-Aldrich) pre-coated 200 μm deep flat carriers (Leica Biosystems), followed by cryo‐immobilization in the EMPACT‐2 HPF apparatus (Leica Microsystems; Vienna Austria) as described ( ).



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